Effects of Plant Bioregulators on the Production of Iridoid Derived Terpenoids in Valeriana wallichii and Fedia comucopiae Cell Suspension Cultures

Four plant bioregulators were tested for their effects on production of valepotriates in Valeriana wallichii and Fedia cornucopiae cell suspension cultures. Concentrations of more than 10 ppm reduced valepotriate yield. A t lower concentrations production was increased. For optimal activi­ ty, bioregulators had to be applied during early exponential growth, up to day 8 of the growth cycle. At equimolar concentrations dimethylmorpholinium bromide (4 ppm) and dimethylpiperidinium chloride (3 ppm) significantly improved total valepotriates in V. wallichii (up to a n d i n F r n r n u r n n in e ( l i n t o 5 0 % ) 4 r l i r h l n r n n h p n n Y v ) t r i > t h y l a m i n f ' ( 6 n n m ) a n r l 2-(3,5-diisopropylphenoxy)-triethylamine (6.4 ppm) increased valepotriate production in both cell cultures up to 40%. With dimethylpiperidinium chloride and dimethylmorpholinium bromide the ratio of m onoene to diene valepotriates in both cell systems was significantly shifted to the monoene compounds. A general use of these bioregulators to increase production of terpenoid secondary m etabolites in plant tissue cultures is indicated.